A simple and efficient strategy to enhance hydrophilicity of zwitterionic<em> </em>sulfoalkylbetaine type monoliths — ASN Events
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  3. A simple and efficient strategy to enhance hydrophilicity of zwitterionic sulfoalkylbetaine type monoliths

A simple and efficient strategy to enhance hydrophilicity of zwitterionic sulfoalkylbetaine type monoliths (#223)

Guangxin Yuan 1 , Jacques Crommen 1 2 , Zhengjin Jiang 1
  1. Department of Pharmacy, Jinan University, Guangzhou, China
  2. Laboratory of Pharmaceutical Analytical Chemistry, Department of Pharmaceutical Sciences, University of Liege, Liege, Belgium

A facile one-step thermally initiated process was developed for the preparation of highly hydrophilic zwitterionic monolithic columns. The novel hydrophilic monomer        N,N-dimethyl-N-acryloyloxyethyl-N-(3-sulfopropyl)ammonium betaine (SPDA) was copolymerized with various crosslinkers, namely 1,4-bis(acryloyl)piperazine (PDA),           N,N’-methylenebisacrylamide (MBA) or ethylene dimethacrylate (EDMA). All monoliths were systematically evaluated based on their permeability, mechanical strength and efficiency. Excellent permeability, reproducibility and stability were achieved on monoliths with optimised composition. It was also noticed that significantly lower critical concentrations of ACN in the mobile phase corresponding to the transition from the HILIC to the RP mode were observed on both poly(SPDA-co-MBA) and poly(SPDA-co-PDA) monoliths. In order to further compare the three kinds of monoliths, their Van Deemter curves and separation selectivity were investigated. An extremely high column efficiency corresponding to about 100,000 plates/m at the optimal velocity was achieved on the poly(SPDA-co-PDA) monoliths. These poly(SPDA-co-PDA) monoliths also exhibited the highest selectivity for a series of polar compounds such as nucleobases, nucleosides, benzoic acid derivatives, phenols and small peptides.