The separation efficiency of steroids on silica bead grafted by varied lengths of thermo-response materials — ASN Events
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  3. The separation efficiency of steroids on silica bead grafted by varied lengths of thermo-response materials

The separation efficiency of steroids on silica bead grafted by varied lengths of thermo-response materials (#173)

Yanli Liang 1 , Zongjian Liu 1 2 3 , Fangfang Geng 1 , Linlin Dong 1 , Rongji Dai 1 , Yulin Deng 1
  1. The School of Life Science, Beijing University of Technology, Beijing, China
  2. Xuanwu Hospital, Capital Medicine University, Beijing, China
  3. The Luhe Teaching Hospital , Capital Medicine University, Beijing, China

Poly (N-isopropylacrylamide) (PNIPAAm)-modified silica bead with varied grafting lengths was prepared via atom transfer radical polymerization (ATRP). The grafting lengths of PNIPAAm on silica surface were controlled by the ratio of composition of the reaction solvent (water and isopropanol). The grafting lengths of PNIPAAm on surface were characterized by thermogravimetric analysis (TGA), and gel permeation chromatography (GPC), respectively. Our results showed that all of these stationary phases exhibit a lower critical solution temperature (LCST) of 32 ºC. Grafting conformation on temperature-responsive property of stationary phase surface was investigated by evaluating the elution behavior of five steroids using water as mobile phase. The results showed that PIPAAm grafting length has a crucial influence on separation efficiency of thermosensitive chromatographic stationary phase. The shorter PNPAAm chains on silica surface, the worse of the thermosensitivity and the degree of seperation. On shorter chain surface, shorter retention time, narrower peak width was observed compared with longer PNIPAAm chain. This is because the difference interaction of the difference analyses with the longer chain is more remarkable compared with the shorter one.

  1. Thermally responsive chromatographicmaterials using functional polymers[J]. J Sep Sci, 2007, 30: 1646-1656.
  2. Development of Novel Thermally Responsible Separation Systems using Functional Polymers[J]. Chromatography, 2009, 30: 1-9.