Characterisation of matrix components which suppress ionisation of Paralytic Shellfish Toxins for analysis with Hydrophilic Interaction Chromatography Tandem Mass Spectrometry — ASN Events
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  2. Chemometrics, method development, optimization and validation
  3. Characterisation of matrix components which suppress ionisation of Paralytic Shellfish Toxins for analysis with Hydrophilic Interaction Chromatography Tandem Mass Spectrometry

Characterisation of matrix components which suppress ionisation of Paralytic Shellfish Toxins for analysis with Hydrophilic Interaction Chromatography Tandem Mass Spectrometry (#32)

Bing Cheng Chai 1 , Rohani Paimin 1 , Katherine Stockham 2
  1. Victoria University, West Melbourne, VIC, Australia
  2. National Measurement Institute, Port Melbourne, VIC, Australia

Paralytic Shellfish Toxins (PST) are a class of neurotoxins which are produced by cyanobacteria and dinoflagellates which bloom seasonally in marine and freshwater environments. Currently, the most used method is the Mouse Bioassay (MBA) which poses ethical and resource challenges. Several Liquid Chromatography Tandem Mass Spectrometer (LCQQQ) methods have been developed, but matrix suppression effects remain a challenge to be overcome. The work presented will focus on efforts to identify and characterise these extract components through LC-QTOF surveys and develop sample preparation and cleanup techniques for potential screening purposes with a Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometer (HILIC MSMS) instrument. 

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