Analysis of BaP in edible oils is challenging owing to its low concentrations relative to the high concentrations of endogenous compounds in the matrix. The aims of pretreatment of oils and fats are removing the triglyceride and tocopherol. The reported methods in bibliography described the use of several general strategies for the sample extraction and clean-up. According to the large conjugated system containing abounded π electronic cloud in PAHs, the utilization of donor–acceptor complex chromatography (DACC) for the cleanup of diluted oil samples is also described. DACC is based on a strong π-π interaction produced between the sorbent and the PAHs; then, certain matrix components, such as neutral lipids and tocopherol, can be eluted by using a non-π-electron containing solvents. After that, the PAHs are eluted with an appropriate organic solvent that removes the interaction (e.g. n-hexane: tetrahydrofuran mixtures, acetonitrile). Compared to traditional methods, this cleanup technique uses less solvent, is less labor intensive, and saves considerable amounts of time. In this article, tetraazacalix[2]arene[2]triazine boned silica was used as a sorbent for the extraction of BaP in oil, which was based on donor-acceptor complex mechanism. The tetraazacalix[2]arene[2]triazine ligand was considered to be the donor of π-electrons, which may form strong π-π interaction with the analytes, while the complicated substrates can be eluted due to weak interactions with the sorbent. After that, the BaP fraction was desorbed with an appropriate solvent. The concentrate was analysed by the HPLC-FLD method. The limits of detection was 0.29 μg/ kg.The recovery of Bap in edible oils were in the range of 85.8%-90.7%, and the intra-day and inter-day relative standard deviations were 2.9% and 3.7%.
　　　
　The authors acknowledge the support of NSF of China (21275133) and State Key Laboratory of Environmental Chemistry & Ecotoxicology (KF2011-30)