In recent years, monoclonal antibodies (mAbs) those are next-generation therapeutic drugs, have been developed and increasingly used for treatment of various diseases such as cancer and rheumatoid arthritis. They are highly specific and are considered to  have fewer adverse events. Unlike low-molecular drugs, antibody preparations are produced by using cultured cells, therefore, detailed and multidimensional analysis of their heterogeneities are required. In this study, we analyzed commercially available five mAb drug preparations by high-temperature reversed-phase LC using a wide pore core-shell column for pluralistic quality assessment. Under highly elevated column temperature, the employed organic solvent with high elurotropic strength coefficients (isopropanol) and wide pore core-shell type octyl column gave good peak resolution of investigated antibody drugs and their related constituents. We have applied this method to estimate a residual rate of intact antibodies after heat aggregation treatment and to simulate fragmentation analysis of them by analyzing their enzymatic digests. Each peak component was identified by MALDI-TOF mass spectrometry. All results were compared with those given by other chromatographic analyses (reversed-phase and size exclusion).