UHPLC technique for determination of biologically active compounds in clinical research — ASN Events
  1. Schedule
  2. Poster Session 2
  3. UHPLC technique for determination of biologically active compounds in clinical research

UHPLC technique for determination of biologically active compounds in clinical research (#259)

Lenka Krcmova 1 2 , Dagmar Solichova 2 , Plisek Jiri 1 2 , Cervinkova Barbora 1 2 , Kucerova Barbora 1 2 , Aufartova Jana 1 2 , Sobotka Luboš 2 , Solich Petr 1
  1. Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Hradec Kralove
  2. University Hospital Hradec Kralove, Hradec Kralove, Czech, Czech Republic

Current clinical research is based on the determination of biologically active compounds which could help to monitore progression of serious diseases and also effectiveness of the treatment.The requirements for biomedical analyses are continuously increasing, therefore the timesaving and effective methods are used more often. The consumption of only small amounts of sample and also of chemicals is required. UHPLC is a new category of analytical separation science that retains the practicality and principles of HPLC, while increasing the attributes of resolution, sensitivity and speed of analysis.
In this work modern UHPLC methods in combination with simple sample preparation procedures for determination of 8OH2dG and liposoluble vitamins A, E and D in biological samples will be presented.
8-hydroxy-2'-deoxyguanosine (8OH2dG) is one of the most common products of DNA damage caused by reactive oxygen species. The levels of 8OH2dG are significantly elevated during various processes, such as aging, neurodegenerative Parkinson's disease and numerous types of malignancies. Liposoluble vitamins A, E and D are native substances which are involved in many biochemical processes in human body. Presented UHPLC methods for monitoring of 8OH2dG, vitamin A, E and 25(OH)D could help to improve treatment of patients, who would benefit from special attention.
Acknowledgement: The work is co-financed by the European Social Fund and the state budget of the Czech Republic, TEAB, project no. CZ.1.07/2.3.00/20.0235 and project IGA MH CR NT 14265-3/2013.