^{Boronate affinity chromatographic plays an important role in proteomics, metabolomics, RNA research for its capcity of specific affinity of cis-glycol structure substances,such as carbohydrates, nucleotides, RNA, glycopeptides, glycoproteins, etc. However, the involvement of switching mobile phase during the elution program limited its use in some extent. The introduction of the temperature-responsive materials may lead to an elution of large biological molecules without changing the mobile phase in affinity chromatography.}

^{Two kinds of thermally switchable bronateaffinity chromatographic materials, Silica@P(NIPAAm-co-DMAEMA-co-VPBA), Silica@P(NIPAAm-co-DMAEMA-co-AAPBA), were prepared by atom transfer radical polymerization (ATRP). Because of the AAPBA owns a lower pKa than VPBA, and the DMAEMA brings a teamed that leading to forming an intermolecular B-N coordination between the amine group and the boronate group. It was founded that the introduction of DMAEMA and AAPBA can decrease the pKa value of boronic acids to increase the affinity at lower pH. The Silica@P(NIPAAm-co-DMAEMA-co-VPBA) modified silica can capture the cis-diol biomolecules at 8.0, and the Silica@P(NIPAAm-co-DMAEMA-co-AAPBA) modified silica  decrease this value to 6.0. The capture/release was achieved by the regulation of surface properties of stationary phase with simpler adjustment to the temperature instead of the conventional method of changing mobile phase. Furthermore, the materials can specially capture the glycopeptides from the trypsin digested peptides of sugar-incubation HSA and HRP.}