This poster provides complementary information to the lecture “Adsorption Isotherm Determination in Supercritical Fluid Chromatography - Pitfalls and Possibilities.
There has been an extensive development in the area of preparative Supercritical Fluid Chromatography (SFC) the last years and many pharmaceutical companies has replaced their preparative Liquid Chromatography (LC) systems with SFC one with higher production rates and that are less harmful to the environment. However, there are still many unresolved fundamental issues, e.g. the advanced theory necessary to correctly model and scale-up SFC is still missing. The compressibility of the mobile phases in SFC generates density, temperature and pressure variations and makes it more complicated to model than LC.

Today there only exist a handful studies that deals with the determination of adsorption isotherms in preparative SFC, and only rarely are the results verified by comparing simulated and experimental overloaded elution profiles. Proper characterization of the adsorption processes is necessary for deeper understanding of analytical and preparative applications and therefore this is a very important area to study.

In this poster we will show how we did the careful system verification, using external sensors of mass flow, temperature and pressure, for the determinations of the adsorption isotherms presented in the corresponding lecture.

This is a contribution from the Fundamental Separation Science Group www.separationscience.se