Hydrophilic interaction chromatography (HILIC) type columns were prepared by attaching some water soluble polymers to silica particles modified with N-(3-triethoxysilylpropyl)methacrylamide (MAS) as anchor. To polymerization, acrylamide (AAm) and acrylic acid (AA) were used in varying amount respectively as monomer substances. Silica particles possessing methacryloyl functionality (MAS-Si) (700 mg), AAm (440, 360, 290, 170 mg) and ammonium persulfate (APS) (23 mg) in phosphate buffer solution (50 mM, pH = 5.8) (5.0 ml) were mixed and warmed at 60 °C for 1h, and poly(AAm) (PAAm)-coated silica particles were obtained. In similar manner, MAS-Si (700 mg), AA (700, 450, 300, 150, 50 mg) and APS (10 mg) in water (5.0 ml) were mixed and warmed at 60 °C for 2h, and PAA-coated ones were obtained. These particles were packed into stainless columns respectively, and 4 PAAm columns and 5 PAA columns were prepared. These columns were tested by the scheme we have previously reported [1] to characterizing their properties of HILIC. On the test scheme, retention factor of uridine shows differences from each other columns even same polymer exclude the degree of polymerization. Polymerization degree, in theory, is associated with monomer concentration. In fact, size exclusion chromatography showed that these molecular weights are different from each other (e.g. PAAm Mp: 400000 - 1200000). These things indicate molecular weight of the polymer moiety relates to retention capacity. In this poster, polymer coverage of silica particles by elemental analysis will also be discussed.