The complementary coupling of liquid chromatography (LC) and capillary electrophoresis (CE) enables comprehensive analysis of complex samples in an orthogonal way. Different components of a mixture may be separated on the basis of either hydrophobic or hydrophilic interactions in LC as the first dimension, and size and charge in CE as the second dimension. The high separation speed of CE addresses the critical requirement for any multidimensional separation, rapidity^1, 2  . With a considerable difference between the peak volume of the LC and the injection volume of the CE, the general key research question answered in this study is related to the adequate interfacing of the two dimensions and the suitable design elements.