T-2 and HT-2 toxin determination in oat contaminated with <em>Fusarium langsethiae</em> in different ecological conditions using an optimized analytical method — ASN Events
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T-2 and HT-2 toxin determination in oat contaminated with Fusarium langsethiae in different ecological conditions using an optimized analytical method (#141)

Josep Esteve-Romero 1 , E.M. Mateo 2 , José-Vicente Gimeno-Adelantado 3 , R. Mateo-Castro 3 , M. Jiménez 2 , Samuel Carda-Broch 1 , Juan Péris Vicente 1
  1. Química Bioanalítica - QFA - ESTCE, Universitat Jaume I, Castelló, La Plana, Spain
  2. Departament de Microbiologia i Ecologia, Universitat de València, València, Sapan
  3. Química Analítica, Universitat de València, València, Spain

Fusarium langsethiae has been isolated from cereals and has been implicated in the production of high levels of T-2 and HT-2 toxins, especially in oats. T-2 toxin is a potent inhibitor of DNA, RNA and protein synthesis. It shows immunosuppressive and cytotoxic effects and induces DNA fragmentation. T-2 is readily metabolized to HT-2 and they induce adverse effects in human and animals with similar potency. The aim of this work was to assess the efficacy of three fungicides on the accumulation of T-2 and HT-2 in oat grains contaminated with Fusarium langsethiae, under different environmental conditions, using an optimized analytical method.

In general, levels of HT-2 were higher than T-2 in all cultures regardless of environmental conditions. HT-2 concentration was higher at 25 ºC than 15 ºC. Levels of both toxins decreased with increasing fungicide dose. No toxins were detected at 0.95 aw in the presence of fungicides. At 0.98 aw neither mycotoxin was found in grains treated with prochloraz at doses >1 mg/kg (15 ºC) or >3 mg/kg (25 ºC) or tebuconazole at doses >6 mg/kg (15 or 25 ºC). ANOVA showed that the factors dose, time and temperature significantly affected toxin production.

Acknowledgements. The authors acknowledge financial support from FEDER and Spanish Government (Project  AGL2010-22182-C04-01-03/ALI) and from Generalitat Valenciana (ACOMP/2013/176). Also, Project P1.1B2012-36 of Pla de Promoció de la Investigació de la Universitat Jaume I