Monitoring the body's concentration of vitamins and their metabolites (e.g. phosphates) is an aid in diagnosing and treating disease states related to their deficiencies. It is important that the analytical method be rapid, sensitive, and accurate in order to accommodate these high-throughput labs that analyse thousands of patient samples each week.  

Vitamins and / or their metabolites were extracted from human plasma by performing a rapid protein precipitation using Impact Protein Precipitation Plates followed by HPLC analysis. HPLC analysis was performed using high efficiency HPLC columns using HPLC-UV, HPLC-FLD or LC/MS/MS systems. The Impact plate allows for the analysis of 96 samples at once, eliminates the transfer steps that are commonly associated with protein precipitation, and can also be automated. Impact technology offers easy, fast protein removal while providing maximized recovery of the target analytes. The HPLC columns used produced excellent chromatographic resolution, sensitivity, and high peak capacities for the analytes.