Purines are biological compounds which have purine rings in their structures. Although purines are essential to living creatures, excess ingestion of them sometimes causes the imbalance of uric acid metabolism. In this study, we established a high-sensitive analytical method for purines in alcohol beverages using hydrophilic interaction chromatography coupled with tandem mass spectrometry (HILIC-MS/MS). The purines in alcohol beverages were hydrolyzed into purine bases (adenine, guanine, hypoxanthine and xanthine) by perchloric acid (60%) and extracted using strong cation exchange solid-phase extraction (Bond Elut SCX). The four purine bases in extracted solution were separated by HILIC with TSKgel Amide-80 as a separation column, 10 mM ammonium formate (pH2) as mobile phase A, and acetonitrile/100 mM ammonium formate (pH2) (90/10) as mobile phase B. The detection of purine bases was performed by MS/MS with ESI. The linearity of calibration curve was 0.997-0.999, and the repeatability was 3.8-5.0% (RSD) for each purine bases. The recovery rates were ranged between 71-116% for a low-purine alcohol beverage, and the detection limits lower than 0.001 mg/100 mL were achieved. This established method is considered to be useful for quality control and surveillance of purine concentration especially in low-purine alcohol beverages.