A novel two dimensional HPLC method (LC-LC-DAD) was developed in this work to determine six food additives simultaneously in yogurt drinks. In this two dimensional liquid chromatography system, a Venusil XBP-C4 analysis column (4.6 mm×100 mm, 5μm, Agela, China) was employed in ¹D as a pre-separation column, a Purospher^® Star RP-18 column (4.6 mm×150 mm, 5μm, Merck, Germany) was empolyed in ²D as a final-analysis column. Fraction transferred from the ¹D to the ²D was performed by means of a two-position six-port switching valve, operated under stop-flow conditions. The whole analytical process is completed in less than 30 minutes without any particular sample preparation procedure. The capability of the new two dimensional HPLC method (LC-LC-DAD) was demonstrated in the determination of six food additives in various brands of yogurt samples using multi-wavelength ultraviolet absorbance detection for the quantitative analysis. To demonstrate the specificity and accuracy of the new LC-LC-DAD method, a referenced one dimensional HPLC method (LC-DAD) was also proposed in this paper. Both of the two methods were validated in terms of linearity, sensitivity, detection limits and separation performance. After the comparison of the results, the benefits of this new LC-LC method were discussed. Compared with the conventional LC-DAD method, the advantages of the new LC-LC-DAD method are as following: (1) this method only required the dissolution of yogurt samples in buffer, which was easy and efficient; (2) it offered higher recovery of each food additive since the loss of analytes can be avoided without using the tedious and complicated sample preparation procedures; (3) the analysis protocol was performed on an automated instrument within 30 min, so the analysis rate is high. Therefore, it is simpler, faster and more accurate than the current used methods which were all based on off-line solid-phase extraction or organic base and heavy metallic salt treatment prior to chromatographic separation.