HPTLC finger print and anti-inflammatory activity of the aqueous extract ofdifferent Maytenus species growing in Saudi Arabia — ASN Events
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  3. HPTLC finger print and anti-inflammatory activity of the aqueous extract ofdifferent Maytenus species growing in Saudi Arabia

HPTLC finger print and anti-inflammatory activity of the aqueous extract ofdifferent Maytenus species growing in Saudi Arabia (#122)

Mohamed AlAjmi 1 , Perwez AlAm 1
  1. King Saud University, Riyadh, Saudi Arabia

Introduction: The genus Maytenus is widely spread in many countries. Members of this genus
are used for treatment of many diseases. This genus is represented in Saudi Arabia by almost
seven plants. Among these plants Maytenus parviflora and Maytenus obscura receive less
attention in the scientific research compared to the other plants.
Objective: To evaluate and compare the anti-inflammatory activity and to develop HPTLC
fingerprint profile of hydroalcoholic extract of Maytenus obscura (MOEE) and Maytenus
parviflora (MPEE). Methods: Preliminary phytochemical screening was done. and HPTLC
studies were carried out using CAMAG HPTLC system equipped with Linomat IV applicator,
TLC scanner 3, Reprostar 3, CAMAG ADC 3 and WIN CATS-4 software. The antiinflammatory
activity was tested by injecting different groups of rats (6 each) with formalin in
hind paw and measuring the edema volume before and 1 hr after formalin injection. Control
group received saline i.p. The extract treatment was injected i.p in doses of 200 and 400mg/kg
1hr before formalin administration. Indomethacin (30 mg/kg) was used as standard. Results:
Treatment of rats (i.p.) with MOEE and MPEE in doses of 200 and 400 mg/kg inhibitied
significantly (p<0.05) formalin-induced inflammation by 55.9%, 63.2% and 77.9%, 82.4%,
respectively. Therefore, preliminary phytochemical studies was done which confirmed the
presence of protein, lipid, carbohydrate, phenol, flavonoid, saponin, triterpenoid, alkaloid and
anthraquinone. Chromatography was performed on glass-backed silica gel 60 F254 HPTLC
plates with the solvent system: Toluene: ethylacetate (EA): glacial acetic acid (GAA) (5:2:0.1,
v/v/v) as mobile phase. HPTLC finger printing of MOEE revealed major 8 peaks with Rf values
in the range of 0.27 to 0.77 and the MPEE revealed maximum 9 peaks with Rf values in the
range of 0.17 to 0.76. The purity of sample was confirmed by comparing the absorption spectra
at start, middle and end position of the band.. Conclusions: HPTLC of MPEE revealed 8 major
spots and 9 spots for MOEE. HPTLC finger printing of hydroalcoholic extract of Maytenus
obscura and Maytenus parviflora may become potential tool for checking authenticity of these
species. It may help in quality control against adulterant and act as a biochemical marker for these medicinally important plant in the pharmaceutical industry and plant systematic studies. The anti-inflammatory potential of these plants also reveals its medicinal importance. It might be further explored for the isolation of phytoconstituents having anti-inflammatory potential.

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  1. [1] Rojo de Almeida MT, Rios-Luci C, Pardόn JM, Palermo, JA. Antiproliferative terpenoids and alkaloids from the roots of Mayenus vitis-idaea and Maytenus spinosa. Phytochemistry 2010; 71(14-15): 1741-1748.
  2. [2] Gutierrez, F, Estévez-Braun A, Ravelo AG, Astudillo L, Zárate R. Terpenoids from the medicinal plant Maytenus ilicifolia. J. Nat. Prod. 2006; 6:1049–1052.
  3. [3] Momtaz S, Hussein AA, Ostad SN, Abbdollahi M, Lall N. Growth inhibition and induction of apoptosis in human cancerous Hela cells by Maytenus procumbens. Food and chemical Toxicology 2013; 51: 38-45.
  4. [4] Crestani S, Rattmann YD, Cipriani TR, De Souza LM, Lacomini M, Kassuya CAL, Marques MCA, Da Silva-Santos JE. A potent and nitiric oxide-dependent hypotensive effect induced in rats by semi-purified fractions from Maytenus ilicifolia. Vascular Pharmacology 2009; 51: 57-63.
  5. [5] Costa PM, Ferreira PMP, Bolzani VS, Furlan M, dos Santos VAFM, Corsino J, de Moraes MO, Costa-Lotufo LV, Montenegro RC, Pessoa C. Antiproliferative activity of pristimerin isolated from Maytenus ilicifolia (Celastraceae) in human HL-60 cells. Toxicol. In Vitro 2008; 22: 854–863.
  6. [6] Bobbarala V, Bramhachari PV, Ravichand J, Reddy YHK, Kotresha D, Chaitanya KV. Evaluation of hydroxyl radical scavenging activity and HPTLC fingerprint profiling of Aegle marmelos (L.) Correa extracts. J Pharm Res 2011; 4(1): 252-255.
  7. [7] Sharma P, Kaushik S, Jain A, Sikarwar SM. Preliminary phytochemical screening and HPTLC fingerprinting of Nicotianatabacum leaf. J Pharm Res 2010; 3(5): 1144-1145. .