HPLC 2013* Single Molecule Adsorption on Chromatographic Surfaces: Theory and Experiment (#25)
The band shapes and migration times of a protein were measured in capillary electrophoresis (CE), and adsorption and desorption events were recorded at the single-molecule level by imaging within the evanescent field layer using total internal reflection fluorescence microscopy. The capacity factor and desorption rate can be estimated from the single-molecule counting results. These agree well with the capacity factor in CE as determined from the mobility-based adsorption isotherms for both computer simulations and experiments. Independently, individual adsorption events can be observed and compared with computer simulations for particles in a cylindrical tube.
