Monitoring Seizure-Induced Oxidative Stress using Liquid Chromatography and Capillary Electrophoresis with Electrochemical and Fluorescence Detection (#49)
The neurotransmitter catecholamines, gamma aminobutyric acid (GABA) and glutamate are important target analytes. A suite of methods using both LC and CE both in combination with either EC or fluorescence have been developed for specific analytical needs. Each of the methods and its uses will be discussed. Several target analytes that serve as biomarkers of oxidative stress have been identified and analytical methods for these targets developed. Glutathione is a key marker of the oxidative state of a tissue. It is necessary to measure both the oxidized (disulfide) and reduced (thiol) forms of glutathione. Again both LC and CE methods using EC detection have been developed. A method that employs on-capillary stacking to achieve acceptable concentration detection limits and a dual-electrode detector will be described. 8-Oxo-guanine (8-oxoG) and 8-hydroxy-deoxyguanosine (8-OHdG) are biomarkers for DNA oxidation. These biomarkers can also be detected using CEEC with on-capillary concentration. Another important biomarker of oxidative stress is malondialdhyde (MDA), which is a product of lipid peroxidation. MDA must be derivatized to a fluorescent product prior to detection using either LC-fourescence or CE-LIF.