After incidents with deliberate addition of melamine to pet food (2007) and milk powder (2008), public awareness on food safety has increased and more efforts are taken by official organizations in order to control quality of food and beverages consumed by humans and animals. In 2012 US FDA listed a number of compounds likely to be used in protein adulteration. Traces of one of these compounds; dicyandiamide or 2-cyanoguanidine, were found in milk products produced by some companies the same year. Dicyandiamide (DC) is a water-soluble, polar and hydrophilic, compound used in the farming process to prevent the fertilizer by-product nitrate entering waterways, and is also used as a means of “promoting pasture growth.” Although there are no international standards for acceptable levels of DC in food products, the news raised immediate fears about the safety of milk, and the local authorities together with affected companies took action. Dicyandiamide has no retention on reversed phase columns, and easily ionize at lower pH values. Until now, there is no standard method for the detection of DC in powder milk in many countries. This poster presents a new fast and easy methodology to detect dicyandiamide in powder milk by either HPLC-UV and/or LC/MS using a SeQuant® ZIC®-HILIC, a bonded zwitterionic hydrophilic interaction liquid chromatography, column. In MS/MS mode the new method showed good linearity over a dynamic range of 5-200 ppb for DC. Several commercially available infant powder milk samples were tested to verify the validity and usefulness of the new methodology.