Optimizing the Separation of 20 Nitro-aromatics Using Consecutively, a Phenyl-Hexyl Column with π-π Interaction and a C-18 Column on the Agilent 1290 Infinity Quaternary Method Development Solution — ASN Events
  1. Schedule
  2. Poster Session 1
  3. Optimizing the Separation of 20 Nitro-aromatics Using Consecutively, a Phenyl-Hexyl Column with π-π Interaction and a C-18 Column on the Agilent 1290 Infinity Quaternary Method Development Solution

Optimizing the Separation of 20 Nitro-aromatics Using Consecutively, a Phenyl-Hexyl Column with π-π Interaction and a C-18 Column on the Agilent 1290 Infinity Quaternary Method Development Solution (#151)

Angelika Gratzfeld-Huesgen 1 , Udo Huber 1
  1. Agilent Technologies, Waldbronn, Germany

The π-π interaction of Phenyl-Hexyl stationary phases with aromatic compounds has been proven by Kirkland and other authors. This poster explores the influence of methanol, acetonitrile, and a mixture of both for the separation of nitro-aromatics on an Agilent Phenyl-Hexyl phase. In addition, the EPA method 8330A/B based on a C18 phase was used for further separation techniques.
Solvent and gradient scouting for the two columns was done using the Agilent 1290 Infinity Quaternary Method Development Solution. Applying the EPA method and the method developed using the Phenyl-Hexyl column, all nitro-aromatics could be separated. A UV spectral library was used to identify the compounds in the different chromatograms. The 1290 Infinity Quaternary Method Development Solution offered the possibility to run both chromatographic methods on a single system, using different columns, mobile phases, and gradients, without the need for manual interaction.